› 3cbt

X-ray diffraction
1.7Å resolution

Crystal structure of SC4828, a unique phosphatase from Streptomyces coelicolor

Released:
DOI: 10.2210/pdb3cbt/pdb
PDB entry 3cbt coloured by chain, front view.
PDB entry 3cbt coloured by chain, side view.
PDB entry 3cbt coloured by chain, top view.
Source organism: Streptomyces coelicolor A3(2)
Entry authors: Singer AU, Xu X, Chang C, Zheng H, Edwards AM, Joachimiak A, Yakunin AF, Savchenko A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190281 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DUF402 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.6 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9FBN7 (Residues: 1-236; Coverage: 100%)
Gene names: SCK7.14c, SCO5041
Sequence domains: Protein of unknown function (DUF402)
Structure domains: FomD-like

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand NA 3 x NA
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 39.738Å b: 77.271Å c: 80.044Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.19 0.215
Expression system: Escherichia coli BL21(DE3)

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