› 3cbw

X-ray diffraction
1.27Å resolution

Crystal structure of the YdhT protein from Bacillus subtilis

Released:
DOI: 10.2210/pdb3cbw/pdb
PDB entry 3cbw coloured by chain, front view.
PDB entry 3cbw coloured by chain, side view.
PDB entry 3cbw coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Bonanno JB, Rutter M, Bain KT, Iizuka M, Romero R, Smith D, Wasserman S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126486 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannan endo-1,4-beta-mannosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 353 amino acids
Theoretical weight: 40.04 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O05512 (Residues: 21-362; Coverage: 100%)
Gene names: BSU05880, gmuG, ydhT
Sequence domains: Glycosyl hydrolase family 26
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand CIT 3 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 57.728Å b: 69.619Å c: 72.505Å
α: 90° β: 91.09° γ: 90°
R-values:
R R work R free
0.151 0.149 0.172
Expression system: Escherichia coli BL21(DE3)

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