› 3cc1

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF A PUTATIVE ALPHA-N-ACETYLGALACTOSAMINIDASE (BH1870) FROM BACILLUS HALODURANS C-125 AT 2.00 A RESOLUTION

Released:
DOI: 10.2210/pdb3cc1/pdb
PDB entry 3cc1 coloured by chain, front view.
PDB entry 3cc1 coloured by chain, side view.
PDB entry 3cc1 coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans C-125
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191825 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-galactosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 433 amino acids
Theoretical weight: 50.38 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KBQ5 (Residues: 1-432; Coverage: 100%)
Gene name: BH1870
Sequence domains:
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand SO4 6 x SO4
Ligand IMD 1 x IMD
Ligand PGE 5 x PGE
Ligand P33 1 x P33
Ligand GOL 5 x GOL
Ligand PEG 6 x PEG
Ligand PG4 1 x PG4
1 modified residue:
Ligand MSE 36 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P6122
Unit cell:
a: 146.943Å b: 146.943Å c: 213.526Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.164 0.162 0.19
Expression system: Escherichia coli

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