› 3ccf

X-ray diffraction
1.9Å resolution

Crystal structure of putative methyltransferase (YP_321342.1) from Anabaena variabilis ATCC 29413 at 1.90 A resolution

Released:
DOI: 10.2210/pdb3ccf/pdb
PDB entry 3ccf coloured by chain, front view.
PDB entry 3ccf coloured by chain, side view.
PDB entry 3ccf coloured by chain, top view.
Source organism: Trichormus variabilis ATCC 29413
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + trans-aconitate = S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174768 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransferase type 11 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 279 amino acids
Theoretical weight: 31.87 KDa
Source organism: Trichormus variabilis ATCC 29413
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3MEY9 (Residues: 1-260; Coverage: 100%)
Gene name: Ava_0823
Sequence domains: Methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

1 bound ligand:
Ligand BEZ 2 x BEZ
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 56.83Å b: 67.23Å c: 131.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.219
Expression system: Escherichia coli

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