› 3ccg

X-ray diffraction
1.5Å resolution

Crystal structure of predicted HD superfamily hydrolase involved in NAD metabolism (NP_347894.1) from Clostridium acetobutylicum at 1.50 A resolution

Released:
DOI: 10.2210/pdb3ccg/pdb
PDB entry 3ccg coloured by chain, front view.
PDB entry 3ccg coloured by chain, side view.
PDB entry 3ccg coloured by chain, top view.
Source organism: Clostridium acetobutylicum ATCC 824
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
P(1),P(4)-bis(5'-adenosyl) tetraphosphate + H(2)O = 2 ADP
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188922 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
bis(5'-nucleosyl)-tetraphosphatase (symmetrical) Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 21.76 KDa
Source organism: Clostridium acetobutylicum ATCC 824
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97JL1 (Residues: 1-189; Coverage: 100%)
Gene name: CA_C1263
Sequence domains: HD domain
Structure domains: Hypothetical protein af1432

Ligands and Environments

2 bound ligands:
Ligand FE 2 x FE
Ligand PO4 1 x PO4
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C2
Unit cell:
a: 90.54Å b: 36.32Å c: 72.6Å
α: 90° β: 125.46° γ: 90°
R-values:
R R work R free
0.182 0.18 0.217
Expression system: Escherichia coli

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