› 3cg4

X-ray diffraction
1.61Å resolution

Crystal structure of response regulator receiver domain protein (CheY-like) from Methanospirillum hungatei JF-1

Released:
DOI: 10.2210/pdb3cg4/pdb
PDB entry 3cg4 coloured by chain, front view.
PDB entry 3cg4 coloured by chain, side view.
PDB entry 3cg4 coloured by chain, top view.
Source organism: Methanospirillum hungatei JF-1
Entry authors: Patskovsky Y, Freeman J, Hu S, Bain K, Smith D, Wasserman SR, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173406 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Response regulatory domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 142 amino acids
Theoretical weight: 16.07 KDa
Source organism: Methanospirillum hungatei JF-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2FQ04 (Residues: 2-132; Coverage: 96%)
Gene name: Mhun_0886
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GOL 2 x GOL
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P65
Unit cell:
a: 69.454Å b: 69.454Å c: 49.14Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.201 0.226
Expression system: Escherichia coli

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