› 3chg

X-ray diffraction
2.8Å resolution

The compatible solute-binding protein OpuAC from Bacillus subtilis in complex with DMSA

Released:
DOI: 10.2210/pdb3chg/pdb
Model geometry
Fit model/data
PDB entry 3chg coloured by chain, front view.
PDB entry 3chg coloured by chain, side view.
PDB entry 3chg coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
The compatible-solute-binding protein OpuAC from Bacillus subtilis: ligand binding, site-directed mutagenesis, and crystallographic studies.
Smits SH, Höing M, Lecher J, Jebbar M, Schmitt L, Bremer E
J Bacteriol 190 5663-71 (2008)
PMID: 18567662

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155534 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycine betaine-binding protein OpuAC Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 268 amino acids
Theoretical weight: 29.78 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P46922 (Residues: 26-293; Coverage: 98%)
Gene names: BSU03000, opuAC
Sequence domains: Substrate binding domain of ABC-type glycine betaine transport system
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 313 4 x 313
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P21
Unit cell:
a: 56.508Å b: 150.606Å c: 58.959Å
α: 90° β: 104.54° γ: 90°
R-values:
R R work R free
0.289 0.285 0.359
Expression system: Escherichia coli

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Citations

4 review citations

A structural classification of substrate-binding proteins.
Berntsson et al. (2010)
3 more

4 mentions without citation

The compatible-solute-binding protein OpuAC from Bacillus subtilis: ligand binding, site-directed mutagenesis, and crystallographic studies.
Smits et al. (2008)
3 more