› 3chm

X-ray diffraction
1.5Å resolution

Crystal structure of PCI domain from A. thaliana COP9 signalosome subunit 7 (CSN7)

Released:
DOI: 10.2210/pdb3chm/pdb
PDB entry 3chm coloured by chain, front view.
PDB entry 3chm coloured by chain, side view.
PDB entry 3chm coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
The Arabidopsis COP9 signalosome subunit 7 is a model PCI domain protein with subdomains involved in COP9 signalosome assembly.
Dessau M, Halimi Y, Erez T, Chomsky-Hecht O, Chamovitz DA, Hirsch JA
Plant Cell 20 2815-34 (2008)
PMID: 18854373

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188238 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
COP9 signalosome complex subunit 7 Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 18.8 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q94JU3 (Residues: 2-169; Coverage: 65%)
Gene names: At1g02090, CSN7, FUS5, T7I23.24, T7I23.25
Sequence domains: PCI domain

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1, ESRF BEAMLINE BM16
Spacegroup: C2221
Unit cell:
a: 57.094Å b: 85.796Å c: 72.343Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.2
Expression system: Escherichia coli

Quick links

Citations

12 review citations

PCI complexes: Beyond the proteasome, CSN, and eIF3 Troika.
Pick et al. (2009)
11 more

11 mentions without citation

Structure characterization of the 26S proteasome.
Kim et al. (2011)
10 more