› 3chx

X-ray diffraction
3.9Å resolution

Crystal structure of Methylosinus trichosporium OB3b particulate methane monooxygenase (pMMO)

Released:
DOI: 10.2210/pdb3chx/pdb
PDB entry 3chx coloured by chain, front view.
PDB entry 3chx coloured by chain, side view.
PDB entry 3chx coloured by chain, top view.
Source organism: Methylosinus trichosporium
Primary publication:
The metal centers of particulate methane monooxygenase from Methylosinus trichosporium OB3b.
Hakemian AS, Kondapalli KC, Telser J, Hoffman BM, Stemmler TL, Rosenzweig AC
Biochemistry 47 6793-801 (2008)
PMID: 18540635

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-163461 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
PmoB Chains: A, E, I
Molecule details ›
Chains: A, E, I
Length: 392 amino acids
Theoretical weight: 43.18 KDa
Source organism: Methylosinus trichosporium
Expression system: Not provided
UniProt:
  • Canonical: Q9KX50 (Residues: 40-431; Coverage: 91%)
Gene name: pmoB
Sequence domains: Monooxygenase subunit B protein
Structure domains:
PmoA Chains: B, F, J
Molecule details ›
Chains: B, F, J
Length: 252 amino acids
Theoretical weight: 28.52 KDa
Source organism: Methylosinus trichosporium
Expression system: Not provided
UniProt:
  • Canonical: Q50541 (Residues: 1-252; Coverage: 100%)
Gene name: pmoA
Sequence domains: Ammonia monooxygenase
Structure domains: Ammonia/particulate methane monooxygenase, subunit A
PmoC Chains: C, G, K
Molecule details ›
Chains: C, G, K
Length: 256 amino acids
Theoretical weight: 29.02 KDa
Source organism: Methylosinus trichosporium
Expression system: Not provided
UniProt:
  • Canonical: Q9KX51 (Residues: 1-256; Coverage: 100%)
Gene name: pmoC
Sequence domains: Ammonia monooxygenase/methane monooxygenase, subunit C
Structure domains: Particulate methane monooxygenase subunit c2. Chain: C
20-residue peptide Chains: D, H, L
Molecule details ›
Chains: D, H, L
Length: 20 amino acids
Theoretical weight: 1.72 KDa
26-residue peptide Chains: M, N, O
Molecule details ›
Chains: M, N, O
Length: 26 amino acids
Theoretical weight: 2.23 KDa

Ligands and Environments

1 bound ligand:
Ligand CU 9 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 113.81Å b: 184.07Å c: 203.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.342 0.342 0.377
Expression system: Not provided

Quick links

Citations

12 review citations

Copper active sites in biology.
Solomon et al. (2014)
11 more

8 mentions without citation

Enzymatic oxidation of methane.
Sirajuddin et al. (2015)
7 more