› 3ci6

X-ray diffraction
1.55Å resolution

Crystal structure of the GAF domain from Acinetobacter phosphoenolpyruvate-protein phosphotransferase

Released:
DOI: 10.2210/pdb3ci6/pdb
PDB entry 3ci6 coloured by chain, front view.
PDB entry 3ci6 coloured by chain, side view.
PDB entry 3ci6 coloured by chain, top view.
Source organism: Acinetobacter baylyi ADP1
Entry authors: Cuff ME, Shackelford G, Kim Y, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179599 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
phosphoenolpyruvate--protein phosphotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 171 amino acids
Theoretical weight: 19.26 KDa
Source organism: Acinetobacter baylyi ADP1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6FEW8 (Residues: 1-168; Coverage: 22%)
Gene names: ACIAD0454, ptsP
Sequence domains: GAF domain
Structure domains: Beta-Lactamase

Ligands and Environments

3 bound ligands:
Ligand P4G 2 x P4G
Ligand PEG 2 x PEG
Ligand GOL 2 x GOL
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 39.081Å b: 54.992Å c: 124.118Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.196
Expression system: Escherichia coli BL21(DE3)

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