› 3cit

X-ray diffraction
1.9Å resolution

Crystal structure of the GAF domain of a putative sensor histidine kinase from Pseudomonas syringae pv. tomato

Released:
DOI: 10.2210/pdb3cit/pdb
PDB entry 3cit coloured by chain, front view.
PDB entry 3cit coloured by chain, side view.
PDB entry 3cit coloured by chain, top view.
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Entry authors: Cuff ME, Li H, Abdullah J, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183416 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sensor histidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 160 amino acids
Theoretical weight: 17.45 KDa
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q884G2 (Residues: 18-174; Coverage: 40%)
Gene name: PSPTO_2131
Structure domains: Beta-Lactamase

Ligands and Environments

4 bound ligands:
Ligand SO4 4 x SO4
Ligand EDO 2 x EDO
Ligand GOL 6 x GOL
Ligand BME 1 x BME
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P3221
Unit cell:
a: 107.471Å b: 107.471Å c: 67.698Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.165 0.2
Expression system: Escherichia coli BL21(DE3)

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