› 3ciw

X-ray diffraction
1.35Å resolution

X-RAY structure of the [FeFe]-hydrogenase maturase HydE from thermotoga maritima

Released:
DOI: 10.2210/pdb3ciw/pdb
PDB entry 3ciw coloured by chain, front view.
PDB entry 3ciw coloured by chain, side view.
PDB entry 3ciw coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
X-ray structure of the [FeFe]-hydrogenase maturase HydE from Thermotoga maritima.
Nicolet Y, Rubach JK, Posewitz MC, Amara P, Mathevon C, Atta M, Fontecave M, Fontecilla-Camps JC
J Biol Chem 283 18861-72 (2008)
PMID: 18400755

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194758 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
[FeFe] hydrogenase maturase subunit HydE Chain: A
Molecule details ›
Chain: A
Length: 348 amino acids
Theoretical weight: 39.91 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X0Z6 (Residues: 1-348; Coverage: 100%)
Gene names: THEMA_07990, TM_1269, Tmari_1274
Sequence domains: Radical SAM superfamily
Structure domains: Aldolase class I

Ligands and Environments

5 bound ligands:
Ligand CL 3 x CL
Ligand SF4 1 x SF4
Ligand SAH 1 x SAH
Ligand CPS 5 x CPS
Ligand SAT 1 x SAT
1 modified residue:
Ligand CSO 2 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 50.974Å b: 79.228Å c: 85.941Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.143 0.177
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

Hydrogenases from methanogenic archaea, nickel, a novel cofactor, and H2 storage.
Thauer et al. (2010)
20 more

12 mentions without citation

Radical S-adenosylmethionine enzymes.
Broderick et al. (2014)
11 more