› 3cjm

X-ray diffraction
1.5Å resolution

Crystal structure of putative beta-lactamase (NP_815223.1) from Enterococcus faecalis V583 at 1.50 A resolution

Released:
DOI: 10.2210/pdb3cjm/pdb
PDB entry 3cjm coloured by chain, front view.
PDB entry 3cjm coloured by chain, side view.
PDB entry 3cjm coloured by chain, top view.
Source organism: Enterococcus faecalis V583
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182806 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 282 amino acids
Theoretical weight: 32.18 KDa
Source organism: Enterococcus faecalis V583
Expression system: Escherichia coli
UniProt:
  • Canonical: Q834X5 (Residues: 33-313; Coverage: 90%)
Gene name: EF_1502
Sequence domains: Beta-lactamase enzyme family
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand NO3 2 x NO3
Ligand EDO 6 x EDO
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: P41212
Unit cell:
a: 64.975Å b: 64.975Å c: 140.905Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.147 0.172
Expression system: Escherichia coli

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