› 3ck5

X-ray diffraction
2.3Å resolution

Crystal structure of a racemase from Streptomyces coelicolor A3(2) with bound magnesium

Released:
DOI: 10.2210/pdb3ck5/pdb
PDB entry 3ck5 coloured by chain, front view.
PDB entry 3ck5 coloured by chain, side view.
PDB entry 3ck5 coloured by chain, top view.
Source organism: Streptomyces coelicolor A3(2)
Entry authors: Rao KN, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
3,6-anhydro-L-galactonate = 2-dehydro-3-deoxy-L-galactonate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo octamer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193189 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3,6-anhydro-alpha-L-galactonate cycloisomerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 371 amino acids
Theoretical weight: 41.21 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RKF7 (Residues: 2-361; Coverage: 100%)
Gene names: SCE65.16c, SCO3480
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 4 x MG
1 modified residue:
Ligand MSE 48 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P4212
Unit cell:
a: 177.603Å b: 177.603Å c: 112.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.264
Expression system: Escherichia coli

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