› 3cl4

X-ray diffraction
2.1Å resolution

Crystal structure of bovine coronavirus hemagglutinin-esterase

Released:
DOI: 10.2210/pdb3cl4/pdb
PDB entry 3cl4 coloured by chain, front view.
PDB entry 3cl4 coloured by chain, side view.
PDB entry 3cl4 coloured by chain, top view.
Source organism: Bovine coronavirus Mebus
Primary publication:
Structure of coronavirus hemagglutinin-esterase offers insight into corona and influenza virus evolution.
Zeng Q, Langereis MA, van Vliet AL, Huizinga EG, de Groot RJ
Proc Natl Acad Sci U S A 105 9065-9 (2008)
PMID: 18550812

Function and Biology Details

Reaction catalysed: 
N-acetyl-O-acetylneuraminate + H(2)O = N-acetylneuraminate + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147480 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Hemagglutinin-esterase Chain: A
Molecule details ›
Chain: A
Length: 377 amino acids
Theoretical weight: 42.65 KDa
Source organism: Bovine coronavirus Mebus
Expression system: Homo sapiens
UniProt:
  • Canonical: P15776 (Residues: 19-388; Coverage: 91%)
Gene names: 2b, HE
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 5 x NAG
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P6522
Unit cell:
a: 88.83Å b: 88.83Å c: 282.36Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.179 0.212
Expression system: Homo sapiens

Quick links

Citations

61 review citations

Viral quasispecies evolution.
Domingo et al. (2012)
60 more

7 mentions without citation

From SARS to MERS: 10 years of research on highly pathogenic human coronaviruses.
Hilgenfeld et al. (2013)
6 more