› 3clc

X-ray diffraction
2.8Å resolution

Crystal Structure of the Restriction-Modification Controller Protein C.Esp1396I Tetramer in Complex with its Natural 35 Base-Pair Operator

Released:
DOI: 10.2210/pdb3clc/pdb
PDB entry 3clc coloured by chain, front view.
PDB entry 3clc coloured by chain, side view.
PDB entry 3clc coloured by chain, top view.
Source organism: Enterobacter sp. RFL1396
Primary publication:
Structural analysis of the genetic switch that regulates the expression of restriction-modification genes.
McGeehan JE, Streeter SD, Thresh SJ, Ball N, Ravelli RB, Kneale GG
Nucleic Acids Res 36 4778-87 (2008)
PMID: 18644840

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116138 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH cro/C1-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 82 amino acids
Theoretical weight: 9.52 KDa
Source organism: Enterobacter sp. RFL1396
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8GGH0 (Residues: 1-79; Coverage: 100%)
Gene name: esp1396IC
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains
35-MER Chain: E
Molecule details ›
Chain: E
Length: 35 nucleotides
Theoretical weight: 10.79 KDa
Source organism: Enterobacter sp. RFL1396
Expression system: Not provided
35-MER Chain: F
Molecule details ›
Chain: F
Length: 35 nucleotides
Theoretical weight: 10.74 KDa
Source organism: Enterobacter sp. RFL1396
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P65
Unit cell:
a: 104.48Å b: 104.48Å c: 139.29Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.207 0.239
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Conflicts targeting epigenetic systems and their resolution by cell death: novel concepts for methyl-specific and other restriction systems.
Ishikawa et al. (2010)
14 other articles

18 mentions without citation

Dissection study on the severe acute respiratory syndrome 3C-like protease reveals the critical role of the extra domain in dimerization of the enzyme: defining the extra domain as a new target for design of highly specific protease inhibitors.
Shi et al. (2004)
17 more