› 3cmg

X-ray diffraction
1.9Å resolution

Crystal structure of putative beta-galactosidase from Bacteroides fragilis

Released:
DOI: 10.2210/pdb3cmg/pdb
PDB entry 3cmg coloured by chain, front view.
PDB entry 3cmg coloured by chain, side view.
PDB entry 3cmg coloured by chain, top view.
Source organism: Bacteroides fragilis NCTC 9343
Entry authors: Ramagopal UA, Rutter M, Toro R, Hu S, Maletic M, Gheyi T, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
A beta-D-glucuronoside + H(2)O = D-glucuronate + an alcohol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177510 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucuronidase Chain: A
Molecule details ›
Chain: A
Length: 667 amino acids
Theoretical weight: 76.69 KDa
Source organism: Bacteroides fragilis NCTC 9343
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5LIC7 (Residues: 38-693; Coverage: 94%)
Gene names: BF9343_0320, NCTC9343_01965, lacZ_4
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand PO4 1 x PO4
Ligand CL 1 x CL
Ligand MPD 3 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A, NSLS BEAMLINE X6A
Spacegroup: I222
Unit cell:
a: 79.651Å b: 102.878Å c: 199.967Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.148 0.186
Expression system: Escherichia coli BL21(DE3)

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