› 3cng

X-ray diffraction
2Å resolution

Crystal structure of NUDIX hydrolase from Nitrosomonas europaea

Released:
DOI: 10.2210/pdb3cng/pdb
PDB entry 3cng coloured by chain, front view.
PDB entry 3cng coloured by chain, side view.
PDB entry 3cng coloured by chain, top view.
Source organism: Nitrosomonas europaea ATCC 19718
Entry authors: Osipiuk J, Xu X, Zheng H, Savchenko A, Edwards AM, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182706 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nudix hydrolase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 189 amino acids
Theoretical weight: 21.85 KDa
Source organism: Nitrosomonas europaea ATCC 19718
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q82VD6 (Residues: 1-185; Coverage: 100%)
Gene name: NE1158
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand ZN 4 x ZN
Ligand NA 2 x NA
Ligand CL 7 x CL
Ligand EDO 3 x EDO
Ligand GOL 4 x GOL
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 174.781Å b: 49.506Å c: 110.19Å
α: 90° β: 106.28° γ: 90°
R-values:
R R work R free
0.183 0.181 0.233
Expression system: Escherichia coli BL21(DE3)

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