› 3cqf

X-ray diffraction
3.1Å resolution

Crystal structure of anthrolysin O (ALO)

Released:
DOI: 10.2210/pdb3cqf/pdb
PDB entry 3cqf coloured by chain, front view.
PDB entry 3cqf coloured by chain, side view.
PDB entry 3cqf coloured by chain, top view.
Source organism: Bacillus anthracis
Primary publication:
Cellular functions and X-ray structure of anthrolysin O, a cholesterol-dependent cytolysin secreted by Bacillus anthracis.
Bourdeau RW, Malito E, Chenal A, Bishop BL, Musch MW, Villereal ML, Chang EB, Mosser EM, Rest RF, Tang WJ
J Biol Chem 284 14645-56 (2009)
PMID: 19307185

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105698 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiol-activated cytolysin Chains: A, B
Molecule details ›
Chains: A, B
Length: 489 amino acids
Theoretical weight: 54.06 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A6L7HHW7 (Residues: 35-512; Coverage: 100%)
Gene names: GBAA_3355, alo
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2221
Unit cell:
a: 141.74Å b: 141.749Å c: 294.099Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.27 0.268 0.294
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Pore-forming toxins: ancient, but never really out of fashion.
Dal Peraro et al. (2016)
17 more

8 mentions without citation

Membrane assembly of the cholesterol-dependent cytolysin pore complex.
Hotze et al. (2012)
7 more