› 3cr1

X-ray diffraction
2.25Å resolution

crystal structure of a minimal, mutant, all-RNA hairpin ribozyme (A38C, A-1OMA) grown from MgCl2

Released:
DOI: 10.2210/pdb3cr1/pdb
Model geometry
Fit model/data
PDB entry 3cr1 coloured by chain, front view.
PDB entry 3cr1 coloured by chain, side view.
PDB entry 3cr1 coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Structural effects of nucleobase variations at key active site residue Ade38 in the hairpin ribozyme.
MacElrevey C, Salter JD, Krucinska J, Wedekind JE
RNA 14 1600-16 (2008)
PMID: 18596253

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-113121 (preferred)
Entry contents:
2 distinct RNA molecules
1 distinct DNA/RNA hybrid molecule
Macromolecules (3 distinct):
RNA (5'-R(*UP*CP*CP*CP*(A2M)P*GP*UP*CP*CP*AP*CP*CP*G)-3') Chain: A
Molecule details ›
Chain: A
Length: 13 nucleotides
Theoretical weight: 4.07 KDa
Source organism: Synthetic construct
Expression system: Not provided
RNA (5'-R(*UP*CP*GP*UP*GP*GP*UP*CP*CP*AP*UP*UP*AP*CP*CP*UP*GP*CP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 19 nucleotides
Theoretical weight: 5.97 KDa
Source organism: Synthetic construct
Expression system: Not provided
loop A and B ribozyme strand Chain: B

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand MG 1 x MG
1 modified residue:
Ligand A2M 1 x A2M

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: CHESS BEAMLINE A1
Spacegroup: P6122
Unit cell:
a: 93.64Å b: 93.64Å c: 133.61Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.219 0.246
Expression system: Not provided

Quick links

Citations

4 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
3 more

1 mention without citation

Metrics for comparison of crystallographic maps.
Urzhumtsev et al. (2014)