› 3cr8

X-ray diffraction
2.95Å resolution

Hexameric APS kinase from Thiobacillus denitrificans

Released:
DOI: 10.2210/pdb3cr8/pdb
PDB entry 3cr8 coloured by chain, front view.
PDB entry 3cr8 coloured by chain, side view.
PDB entry 3cr8 coloured by chain, top view.
Source organism: Thiobacillus denitrificans
Primary publication:
Structure of the two-domain hexameric APS kinase from Thiobacillus denitrificans: structural basis for the absence of ATP sulfurylase activity.
Gay SC, Segel IH, Fisher AJ
Acta Crystallogr D Biol Crystallogr 65 1021-31 (2009)
PMID: 19770499

Function and Biology Details

Reaction catalysed: 
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174788 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylyl-sulfate kinase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 552 amino acids
Theoretical weight: 61.64 KDa
Source organism: Thiobacillus denitrificans
Expression system: Thiobacillus denitrificans ATCC 25259
UniProt:
  • Canonical: Q3SM86 (Residues: 1-544; Coverage: 100%)
Gene names: Tbd_0210, cysC
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2221
Unit cell:
a: 161.071Å b: 227.211Å c: 106.766Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.243 0.282
Expression system: Thiobacillus denitrificans ATCC 25259

Quick links

Citations

2 review citations

Sulfation pathways from red to green.
Günal et al. (2019)
1 more

5 mentions without citation

Structure of the two-domain hexameric APS kinase from Thiobacillus denitrificans: structural basis for the absence of ATP sulfurylase activity.
Gay et al. (2009)
4 more