› 3crc

X-ray diffraction
3Å resolution

Crystal Structure of Escherichia coli MazG, the Regulator of Nutritional Stress Response

Released:
DOI: 10.2210/pdb3crc/pdb
PDB entry 3crc coloured by chain, front view.
PDB entry 3crc coloured by chain, side view.
PDB entry 3crc coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structure of Escherichia coli MazG, the regulator of nutritional stress response.
Lee S, Kim MH, Kang BS, Kim JS, Kim GH, Kim YG, Kim KJ
J Biol Chem 283 15232-40 (2008)
PMID: 18353782

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = AMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142668 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoside triphosphate pyrophosphohydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 265 amino acids
Theoretical weight: 30.59 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEY3 (Residues: 1-263; Coverage: 100%)
Gene names: JW2752, b2781, mazG
Sequence domains: MazG nucleotide pyrophosphohydrolase domain
Structure domains: MazG-like

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6C1
Spacegroup: P212121
Unit cell:
a: 63.23Å b: 66.91Å c: 140.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.216 0.345
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Direct binding targets of the stringent response alarmone (p)ppGpp.
Kanjee et al. (2012)
1 more

4 mentions without citation

Crystal structure of Escherichia coli MazG, the regulator of nutritional stress response.
Lee et al. (2008)
3 more