› 3cri

X-ray diffraction
2.1Å resolution

Crystal structure of human fibroblast growth factor-1 with mutations Glu81Ser, Glu82Asn and Lys101Ala

Released:
DOI: 10.2210/pdb3cri/pdb
PDB entry 3cri coloured by chain, front view.
PDB entry 3cri coloured by chain, side view.
PDB entry 3cri coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Engineering an improved crystal contact across a solvent-mediated interface of human fibroblast growth factor 1.
Meher AK, Blaber SI, Lee J, Honjo E, Kuroki R, Blaber M
Acta Crystallogr Sect F Struct Biol Cryst Commun 65 1136-40 (2009)
PMID: 19923735

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fibroblast growth factor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 146 amino acids
Theoretical weight: 16.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05230 (Residues: 16-155; Coverage: 90%)
Gene names: FGF1, FGFA
Sequence domains: Fibroblast growth factor
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand FMT 2 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: C2221
Unit cell:
a: 77.86Å b: 93.33Å c: 107.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.228
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Mapping the conformational space accessible to BACE2 using surface mutants and cocrystals with Fab fragments, Fynomers and Xaperones.
Banner et al. (2013)

1 mention without citation

Engineering an improved crystal contact across a solvent-mediated interface of human fibroblast growth factor 1.
Meher et al. (2009)