Function and Biology Details
Reactions catalysed:
L-seryl-[pyruvate dehydrogenase E1 alpha subunit] + ATP = O-phospho-L-seryl-[pyruvate dehydrogenase E1 alpha subunit] + ADP + H(+).
N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + acetyl-CoA = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CoA.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Biotin/lipoyl attachment
- Histidine kinase/HSP90-like ATPase domain
- Histidine kinase/HSP90-like ATPase superfamily
- Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain superfamily
- 2-oxo acid dehydrogenase, lipoyl-binding site
- Dihydrolipoamide acetyltransferase/Pyruvate dehydrogenase protein X component
- Single hybrid motif
- Histidine kinase domain
2 more domains
Structure analysis Details
Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145329 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial
Molecule details ›
Chains: A, B
Length: 407 amino acids
Theoretical weight: 46.16 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 407 amino acids
Theoretical weight: 46.16 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q64536 (Residues: 1-407; Coverage: 100%)
Sequence domains:
- Mitochondrial branched-chain alpha-ketoacid dehydrogenase kinase
- Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
Molecule details ›
Chains: C, D
Length: 87 amino acids
Theoretical weight: 9.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Biotin-requiring enzyme
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Length: 87 amino acids
Theoretical weight: 9.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P10515 (Residues: 214-300; Coverage: 13%)
Sequence domains: Biotin-requiring enzyme
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
