› 3crn

X-ray diffraction
1.58Å resolution

Crystal structure of response regulator receiver domain protein (CheY-like) from Methanospirillum hungatei JF-1

Released:
DOI: 10.2210/pdb3crn/pdb
PDB entry 3crn coloured by chain, front view.
PDB entry 3crn coloured by chain, side view.
PDB entry 3crn coloured by chain, top view.
Source organism: Methanospirillum hungatei JF-1
Entry authors: Hiner RL, Toro R, Patskovsky Y, Freeman J, Chang S, Smith D, Groshong C, Wasserman SR, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173405 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Response regulatory domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 132 amino acids
Theoretical weight: 15.27 KDa
Source organism: Methanospirillum hungatei JF-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2FNF5 (Residues: 2-122; Coverage: 85%)
Gene name: Mhun_3201
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand NA 4 x NA
Ligand GOL 4 x GOL
2 modified residues:
Ligand MSE 10 x MSE
Ligand PHD 2 x PHD

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 51.93Å b: 53.992Å c: 92.681Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.224
Expression system: Escherichia coli

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