3cs3

X-ray diffraction
2.4Å resolution

Crystal structure of sugar-binding transcriptional regulator (LacI family) from Enterococcus faecalis

Released:
Source organism: Enterococcus faecalis V583
Entry authors: Patskovsky Y, Romero R, Freeman J, Iizuka M, Groshong C, Smith D, Wasserman SR, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-182817 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH lacI-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 31.33 KDa
Source organism: Enterococcus faecalis V583
Expression system: Escherichia coli
UniProt:
  • Canonical: Q835X8 (Residues: 55-320; Coverage: 83%)
Gene name: EF_1240
Sequence domains: Periplasmic binding protein-like domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P6522
Unit cell:
a: 93.25Å b: 93.25Å c: 158.02Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.204 0.249
Expression system: Escherichia coli