› 3csw

X-ray diffraction
2.15Å resolution

Crystal structure of a putative branched-chain amino acid aminotransferase (TM0831) from Thermotoga maritima at 2.15 A resolution

Released:
DOI: 10.2210/pdb3csw/pdb
PDB entry 3csw coloured by chain, front view.
PDB entry 3csw coloured by chain, side view.
PDB entry 3csw coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159964 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable branched-chain-amino-acid aminotransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 285 amino acids
Theoretical weight: 32.76 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: P74921 (Residues: 1-273; Coverage: 100%)
Gene names: TM_0831, ilvE
Sequence domains: Amino-transferase class IV
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
4 bound ligands:
Ligand UNL 4 x UNL
Ligand CIT 3 x CIT
Ligand MPD 7 x MPD
Ligand CL 4 x CL
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: I212121
Unit cell:
a: 107.71Å b: 129.76Å c: 298.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.167 0.2
Expression system: Escherichia coli

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