› 3csz

X-ray diffraction
1.8Å resolution

Crystal and cryoEM structural studies of a cell wall degrading enzyme in the bacteriophage phi29 tail

Released:
DOI: 10.2210/pdb3csz/pdb
PDB entry 3csz coloured by chain, front view.
PDB entry 3csz coloured by chain, side view.
PDB entry 3csz coloured by chain, top view.
Source organism: Bacillus phage phi29
Primary publication:
Crystal and cryoEM structural studies of a cell wall degrading enzyme in the bacteriophage phi29 tail.
Xiang Y, Morais MC, Cohen DN, Bowman VD, Anderson DL, Rossmann MG
Proc Natl Acad Sci U S A 105 9552-7 (2008)
PMID: 18606992

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147227 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Morphogenesis protein 1 Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 18.44 KDa
Source organism: Bacillus phage phi29
Expression system: Escherichia coli
UniProt:
  • Canonical: P15132 (Residues: 1-159; Coverage: 44%)
Gene name: 13
Sequence domains: Phage tail lysozyme
Structure domains: Lysozyme

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 30.876Å b: 69.35Å c: 70.728Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.183 0.225
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Molecular architecture of tailed double-stranded DNA phages.
Fokine et al. (2014)
10 more

1 mention without citation

Crystal and cryoEM structural studies of a cell wall degrading enzyme in the bacteriophage phi29 tail.
Xiang et al. (2008)