› 3ct6

X-ray diffraction
1.1Å resolution

Crystal structure of DhaM of L. lactis

Released:
DOI: 10.2210/pdb3ct6/pdb
Model geometry
Fit model/data
PDB entry 3ct6 coloured by chain, front view.
PDB entry 3ct6 coloured by chain, side view.
PDB entry 3ct6 coloured by chain, top view.
Source organism: Lactococcus lactis
Primary publication:
X-ray structures of the three Lactococcus lactis dihydroxyacetone kinase subunits and of a transient intersubunit complex.
Zurbriggen A, Jeckelmann JM, Christen S, Bieniossek C, Baumann U, Erni B
J Biol Chem 283 35789-96 (2008)
PMID: 18957416

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + glycerone = pyruvate + glycerone phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189974 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS-dependent dihydroxyacetone kinase, phosphotransferase subunit DhaM Chains: A, B
Molecule details ›
Chains: A, B
Length: 131 amino acids
Theoretical weight: 14.48 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9CIV6 (Residues: 1-123; Coverage: 100%)
Gene names: L47257, LL0250, dhaM
Sequence domains: PTS system fructose IIA component
Structure domains: Phosphotransferase system, mannose-type IIA component

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: P21
Unit cell:
a: 30.6Å b: 62.4Å c: 69.7Å
α: 90° β: 99.6° γ: 90°
R-values:
R R work R free
0.146 not available 0.177
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Glycerol metabolism of haloarchaea.
Williams et al. (2017)
12 other articles