› 3ctk

X-ray diffraction
1.8Å resolution

Crystal structure of the type 1 RIP bouganin

Released:
DOI: 10.2210/pdb3ctk/pdb
PDB entry 3ctk coloured by chain, front view.
PDB entry 3ctk coloured by chain, side view.
PDB entry 3ctk coloured by chain, top view.
Source organism: Bougainvillea spectabilis
Primary publication:
Structure/function studies on two type 1 ribosome inactivating proteins: Bouganin and lychnin.
Fermani S, Tosi G, Farini V, Polito L, Falini G, Ripamonti A, Barbieri L, Chambery A, Bolognesi A
J Struct Biol 168 278-87 (2009)
PMID: 19616098

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186665 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
rRNA N-glycosylase Chain: A
Molecule details ›
Chain: A
Length: 248 amino acids
Theoretical weight: 27.8 KDa
Source organism: Bougainvillea spectabilis
UniProt:
  • Canonical: Q8W4U4 (Residues: 27-274; Coverage: 81%)
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID13
Spacegroup: P212121
Unit cell:
a: 40.838Å b: 77.501Å c: 79.383Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 0.202

Quick links

Citations

6 review citations

Saporin-S6: a useful tool in cancer therapy.
Polito et al. (2013)
5 more

7 mentions without citation

Ribosome-inactivating proteins: from plant defense to tumor attack.
de Virgilio et al. (2010)
6 more