› 3ctv

X-ray diffraction
2.46Å resolution

Crystal structure of central domain of 3-hydroxyacyl-CoA dehydrogenase from Archaeoglobus fulgidus

Released:
DOI: 10.2210/pdb3ctv/pdb
PDB entry 3ctv coloured by chain, front view.
PDB entry 3ctv coloured by chain, side view.
PDB entry 3ctv coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Entry authors: Osipiuk J, Evdokimova E, Kudritska M, Savchenko A, Edwards AM, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127927 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
enoyl-CoA hydratase Chain: A
Molecule details ›
Chain: A
Length: 110 amino acids
Theoretical weight: 12.17 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O28011 (Residues: 295-400; Coverage: 16%)
Gene name: AF_2273
Sequence domains: 3-hydroxyacyl-CoA dehydrogenase, C-terminal domain
Structure domains: N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase; domain 2

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: I213
Unit cell:
a: 101.464Å b: 101.464Å c: 101.464Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 0.248
Expression system: Escherichia coli BL21(DE3)

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