› 3cuo

X-ray diffraction
2Å resolution

Crystal structure of the predicted DNA-binding transcriptional regulator from E. coli

Released:
DOI: 10.2210/pdb3cuo/pdb
PDB entry 3cuo coloured by chain, front view.
PDB entry 3cuo coloured by chain, side view.
PDB entry 3cuo coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Zhang R, Evdokimova E, Kagan O, Savchenko A, Edwards AM, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160145 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable HTH-type transcriptional regulator YgaV Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 99 amino acids
Theoretical weight: 10.61 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P77295 (Residues: 1-99; Coverage: 100%)
Gene names: JW2642, b2667, ygaV
Sequence domains: Bacterial regulatory protein, arsR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 39.994Å b: 39.632Å c: 56.279Å
α: 95.93° β: 95.78° γ: 97.41°
R-values:
R R work R free
0.231 0.227 0.289
Expression system: Escherichia coli BL21

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