› 3cup

X-ray diffraction
3.09Å resolution

Crystal structure of the MHC class II molecule I-Ag7 in complex with the peptide GAD221-235

Released:
DOI: 10.2210/pdb3cup/pdb
PDB entry 3cup coloured by chain, front view.
PDB entry 3cup coloured by chain, side view.
PDB entry 3cup coloured by chain, top view.
Source organisms:
Entry authors: Corper AL, Yoshida K, Teyton L, Wilson IA

Function and Biology Details

Reaction catalysed: 
L-glutamate = 4-aminobutanoate + CO(2)
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137524 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
H-2 class II histocompatibility antigen, A-D alpha chain Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 21.62 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P04228 (Residues: 24-205; Coverage: 78%)
Gene name: H2-Aa
Sequence domains:
Structure domains:
Glutamate decarboxylase 2; Ig-like domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 222 amino acids
Theoretical weight: 25.54 KDa
Source organisms: Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q31135 (Residues: 20-20, 28-214; Coverage: 76%)
  • Canonical: Q05683 (Residues: 222-235; Coverage: 2%)
Gene names: Gad2, Gad65, H2-Ab1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NAG 1 x NAG
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P43212
Unit cell:
a: 55.832Å b: 55.832Å c: 338.834Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.232 0.286
Expression system: Drosophila melanogaster

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