› 3cw2

X-ray diffraction
2.8Å resolution

Crystal structure of the intact archaeal translation initiation factor 2 from Sulfolobus solfataricus .

Released:
DOI: 10.2210/pdb3cw2/pdb
PDB entry 3cw2 coloured by chain, front view.
PDB entry 3cw2 coloured by chain, side view.
PDB entry 3cw2 coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Crystal structure of the intact archaeal translation initiation factor 2 demonstrates very high conformational flexibility in the alpha- and beta-subunits.
Stolboushkina E, Nikonov S, Nikulin A, Bläsi U, Manstein DJ, Fedorov R, Garber M, Nikonov O
J Mol Biol 382 680-91 (2008)
PMID: 18675278

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189003 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Translation initiation factor 2 subunit gamma Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 415 amino acids
Theoretical weight: 45.85 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q980A5 (Residues: 1-415; Coverage: 100%)
Gene names: SSO0412, eif2g
Sequence domains:
Structure domains:
Translation initiation factor 2 subunit alpha Chains: C, D, G, H
Molecule details ›
Chains: C, D, G, H
Length: 266 amino acids
Theoretical weight: 30.43 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97Z79 (Residues: 1-266; Coverage: 100%)
Gene names: SSO1050, aif2a, eif2a
Sequence domains:
Structure domains:
Translation initiation factor 2 subunit beta Chains: K, L, M, N
Molecule details ›
Chains: K, L, M, N
Length: 139 amino acids
Theoretical weight: 15.94 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97W59 (Residues: 1-139; Coverage: 100%)
Gene names: SSO2381, aif2b, eif2b
Sequence domains: Domain found in IF2B/IF5

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: P21
Unit cell:
a: 79.2Å b: 162.92Å c: 161.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.276
Expression system: Escherichia coli

Quick links

Citations

12 review citations

The scanning mechanism of eukaryotic translation initiation.
Hinnebusch AG. (2014)
11 more

11 mentions without citation

Molecular view of 43 S complex formation and start site selection in eukaryotic translation initiation.
Lorsch et al. (2010)
10 more