› 3cw4

X-ray diffraction
2.7Å resolution

Large c-terminal domain of influenza a virus RNA-dependent polymerase PB2

Released:
DOI: 10.2210/pdb3cw4/pdb
PDB entry 3cw4 coloured by chain, front view.
PDB entry 3cw4 coloured by chain, side view.
PDB entry 3cw4 coloured by chain, top view.
Source organism: Influenza A virus
Primary publication:
Structural basis of the influenza A virus RNA polymerase PB2 RNA-binding domain containing the pathogenicity-determinant lysine 627 residue.
Kuzuhara T, Kise D, Yoshida H, Horita T, Murazaki Y, Nishimura A, Echigo N, Utsunomiya H, Tsuge H
J Biol Chem 284 6855-60 (2009)
PMID: 19144639

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137006 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase basic protein 2 Chain: A
Molecule details ›
Chain: A
Length: 225 amino acids
Theoretical weight: 25.3 KDa
Source organism: Influenza A virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P03428 (Residues: 535-759; Coverage: 30%)
Gene name: PB2
Sequence domains:
Structure domains: Polymerase Basic Protein 2, C-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P3221
Unit cell:
a: 52.527Å b: 52.527Å c: 156.357Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.24 0.237 0.29
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Emergence and pandemic potential of swine-origin H1N1 influenza virus.
Neumann et al. (2009)
15 more

8 mentions without citation

Biological and structural characterization of a host-adapting amino acid in influenza virus.
Yamada et al. (2010)
7 more