› 3cw5

X-ray diffraction
3.1Å resolution

E. coli Initiator tRNA

Released:
DOI: 10.2210/pdb3cw5/pdb
PDB entry 3cw5 coloured by chain, front view.
PDB entry 3cw5 coloured by chain, side view.
PDB entry 3cw5 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A unique conformation of the anticodon stem-loop is associated with the capacity of tRNAfMet to initiate protein synthesis.
Barraud P, Schmitt E, Mechulam Y, Dardel F, Tisné C
Nucleic Acids Res 36 4894-901 (2008)
PMID: 18653533

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195823 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
Initiator tRNA Chain: A
Molecule details ›
Chain: A
Length: 77 nucleotides
Theoretical weight: 24.83 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Sequence domains: tRNA

Ligands and Environments

No bound ligands
5 modified residues:
Ligand 4SU 1 x 4SU
Ligand H2U 1 x H2U
Ligand OMC 1 x OMC
Ligand 5MU 1 x 5MU
Ligand PSU 1 x PSU

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P6422
Unit cell:
a: 93.79Å b: 93.79Å c: 219.36Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.244 0.241 0.271
Expression system: Escherichia coli

Quick links

Citations

6 review citations

An integrated, structure- and energy-based view of the genetic code.
Grosjean et al. (2016)
5 more

14 mentions without citation

m1A Post-Transcriptional Modification in tRNAs.
Oerum et al. (2017)
13 more