› 3cwf

X-ray diffraction
2.2Å resolution

Crystal structure of PAS domain of two-component sensor histidine kinase

Released:
DOI: 10.2210/pdb3cwf/pdb
PDB entry 3cwf coloured by chain, front view.
PDB entry 3cwf coloured by chain, side view.
PDB entry 3cwf coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Extracytoplasmic PAS-like domains are common in signal transduction proteins.
Chang C, Tesar C, Gu M, Babnigg G, Joachimiak A, Pokkuluri PR, Szurmant H, Schiffer M
J Bacteriol 192 1156-9 (2010)
PMID: 20008068

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150008 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkaline phosphatase synthesis sensor protein PhoR Chains: A, B
Molecule details ›
Chains: A, B
Length: 122 amino acids
Theoretical weight: 12.92 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: P23545 (Residues: 32-150; Coverage: 21%)
Gene names: BSU29100, phoR
Sequence domains: Single Cache-like
Structure domains: PAS domain

Ligands and Environments

2 bound ligands:
Ligand EPE 2 x EPE
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 55.503Å b: 67.632Å c: 77.695Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.244
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Ligand-binding PAS domains in a genomic, cellular, and structural context.
Henry et al. (2011)
5 more

10 mentions without citation

Structure and signaling mechanism of Per-ARNT-Sim domains.
Möglich et al. (2009)
9 more