› 3cwi

X-ray diffraction
1.9Å resolution

Crystal structure of thiamine biosynthesis protein (ThiS) from Geobacter metallireducens. Northeast Structural Genomics Consortium Target GmR137

Released:
DOI: 10.2210/pdb3cwi/pdb
PDB entry 3cwi coloured by chain, front view.
PDB entry 3cwi coloured by chain, side view.
PDB entry 3cwi coloured by chain, top view.
Source organism: Geobacter metallireducens GS-15
Entry authors: Forouhar F, Abashidze M, Seetharaman J, Mao L, Janjua H, Xiao R, Maglaqui M, Ciccosanti C, Foote EL, Wang H, Everett JK, Acton TB, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174481 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiamin biosynthesis sulfur carrier protein Chain: A
Molecule details ›
Chain: A
Length: 78 amino acids
Theoretical weight: 8.62 KDa
Source organism: Geobacter metallireducens GS-15
Expression system: Escherichia coli
UniProt:
  • Canonical: Q39VC5 (Residues: 1-70; Coverage: 100%)
Gene names: Gmet_1567, thiS-2
Sequence domains: ThiS family
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P43212
Unit cell:
a: 40.965Å b: 40.965Å c: 89.537Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.226 0.258
Expression system: Escherichia coli

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