› 3cwv

X-ray diffraction
1.95Å resolution

Crystal structure of B-subunit of the DNA gyrase from Myxococcus xanthus

Released:
DOI: 10.2210/pdb3cwv/pdb
PDB entry 3cwv coloured by chain, front view.
PDB entry 3cwv coloured by chain, side view.
PDB entry 3cwv coloured by chain, top view.
Source organism: Myxococcus xanthus DK 1622
Entry authors: Ramagopal UA, Toro R, Meyer AJ, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172786 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase (ATP-hydrolyzing) Chains: A, B
Molecule details ›
Chains: A, B
Length: 369 amino acids
Theoretical weight: 40.54 KDa
Source organism: Myxococcus xanthus DK 1622
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q1CZR7 (Residues: 3-360; Coverage: 99%)
Gene name: MXAN_5971
Sequence domains: DNA gyrase B
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 160.606Å b: 57.799Å c: 119.437Å
α: 90° β: 127.23° γ: 90°
R-values:
R R work R free
0.211 0.209 0.247
Expression system: Escherichia coli BL21(DE3)

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