› 3cxm

X-ray diffraction
1.5Å resolution

Leishmania naiffi uracil-DNA glycosylase in complex with 5-bromouracil

Released:
DOI: 10.2210/pdb3cxm/pdb
PDB entry 3cxm coloured by chain, front view.
PDB entry 3cxm coloured by chain, side view.
PDB entry 3cxm coloured by chain, top view.
Source organism: Leishmania naiffi
Entry authors: Larson ET, Merritt EA, Structural Genomics of Pathogenic Protozoa Consortium (SGPP)

Function and Biology Details

Reaction catalysed: 
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112098 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uracil-DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 268 amino acids
Theoretical weight: 30.29 KDa
Source organism: Leishmania naiffi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D0VWU0 (Residues: 16-260; Coverage: 94%)
Sequence domains: Uracil DNA glycosylase superfamily
Structure domains: Uracil-DNA glycosylase-like domain

Ligands and Environments

4 bound ligands:
Ligand BR 1 x BR
Ligand URB 1 x URB
Ligand URA 1 x URA
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P61
Unit cell:
a: 75.32Å b: 75.32Å c: 105.703Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.139 0.138 0.16
Expression system: Escherichia coli BL21(DE3)

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