› 3cxu

X-ray diffraction
2Å resolution

Structure of a Y149F mutant of epoxide hydrolase from Solanum tuberosum

Released:
DOI: 10.2210/pdb3cxu/pdb
PDB entry 3cxu coloured by chain, front view.
PDB entry 3cxu coloured by chain, side view.
PDB entry 3cxu coloured by chain, top view.
Source organism: Solanum tuberosum
Primary publication:
Removal of distal protein-water hydrogen bonds in a plant epoxide hydrolase increases catalytic turnover but decreases thermostability.
Thomaeus A, Naworyta A, Mowbray SL, Widersten M
Protein Sci 17 1275-84 (2008)
PMID: 18515642

Function and Biology Details

Reaction catalysed: 
An epoxide + H(2)O = a glycol
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174947 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 328 amino acids
Theoretical weight: 37.14 KDa
Source organism: Solanum tuberosum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q41415 (Residues: 1-321; Coverage: 100%)
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 55.969Å b: 96.749Å c: 122.215Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.203
Expression system: Escherichia coli

Quick links

Citations

10 citation in other articles

Engineering of an epoxide hydrolase for efficient bioresolution of bulky pharmaco substrates.
Kong et al. (2014)
9 more

2 mentions without citation

Expanding the Catalytic Triad in Epoxide Hydrolases and Related Enzymes.
Amrein et al. (2015)
1 more