› 3cxy

X-ray diffraction
1.45Å resolution

Crystal structure of the cytochrome P450 CYP121 P346L mutant from M. tuberculosis

Released:
DOI: 10.2210/pdb3cxy/pdb
PDB entry 3cxy coloured by chain, front view.
PDB entry 3cxy coloured by chain, side view.
PDB entry 3cxy coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Entry author: Leys D

Function and Biology Details

Reaction catalysed: 
Cyclo(L-tyrosyl-L-tyrosyl) + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = mycocyclosin + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162004 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mycocyclosin synthase Chain: A
Molecule details ›
Chain: A
Length: 396 amino acids
Theoretical weight: 43.32 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WPP7 (Residues: 1-396; Coverage: 100%)
Gene names: MTCY339.34c, Rv2276, cyp121
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P6522
Unit cell:
a: 77.714Å b: 77.714Å c: 265.046Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.154 0.152 0.186
Expression system: Escherichia coli

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