› 3cza

X-ray diffraction
1.2Å resolution

Crystal Structure of E18D DJ-1

Released:
DOI: 10.2210/pdb3cza/pdb
PDB entry 3cza coloured by chain, front view.
PDB entry 3cza coloured by chain, side view.
PDB entry 3cza coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cysteine pKa depression by a protonated glutamic acid in human DJ-1.
Witt AC, Lakshminarasimhan M, Remington BC, Hasim S, Pozharski E, Wilson MA
Biochemistry 47 7430-40 (2008)
PMID: 18570440

Function and Biology Details

Reaction catalysed: 
An N(6)-(1-hydroxy-2-oxopropyl)-[protein]-L-lysine + H(2)O = a [protein]-L-lysine + lactate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189207 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Parkinson disease protein 7 Chain: A
Molecule details ›
Chain: A
Length: 197 amino acids
Theoretical weight: 20.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99497 (Residues: 1-189; Coverage: 100%)
Gene name: PARK7
Sequence domains: DJ-1/PfpI family
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand MLA 1 x MLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P3121
Unit cell:
a: 74.826Å b: 74.826Å c: 74.777Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.13 0.13 0.164
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Cysteine-mediated redox signaling: chemistry, biology, and tools for discovery.
Paulsen et al. (2013)
14 more

2 mentions without citation

Sulfenic acid chemistry, detection and cellular lifetime.
Gupta et al. (2014)
1 more