› 3d0a

X-ray diffraction
1.8Å resolution

Human p53 core domain with hot spot mutation R249S and second site suppressor mutation H168R in sequence-specific complex with DNA

Released:
DOI: 10.2210/pdb3d0a/pdb
PDB entry 3d0a coloured by chain, front view.
PDB entry 3d0a coloured by chain, side view.
PDB entry 3d0a coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of restoring sequence-specific DNA binding and transactivation to mutant p53 by suppressor mutations.
Suad O, Rozenberg H, Brosh R, Diskin-Posner Y, Kessler N, Shimon LJ, Frolow F, Liran A, Rotter V, Shakked Z
J Mol Biol 385 249-65 (2009)
PMID: 18996393

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116165 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Cellular tumor antigen p53 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 200 amino acids
Theoretical weight: 22.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04637 (Residues: 94-293; Coverage: 51%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like
DNA (5'-D(*DCP*DGP*DGP*DGP*DCP*DAP*DTP*DGP*DCP*DCP*DCP*DG)-3') Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P1
Unit cell:
a: 54.299Å b: 57.986Å c: 78.926Å
α: 83.05° β: 88.11° γ: 74.08°
R-values:
R R work R free
0.186 0.183 0.241
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

8 review citations

Mutant p53: one name, many proteins.
Freed-Pastor et al. (2012)
7 more

4 mentions without citation

Chemical Decorations of "MARs" Residents in Orchestrating Eukaryotic Gene Regulation.
Roychowdhury et al. (2020)
3 more