› 3d0w

X-ray diffraction
2Å resolution

Crystal structure of YflH protein from Bacillus subtilis. Northeast Structural Genomics Consortium target SR326

Released:
DOI: 10.2210/pdb3d0w/pdb
PDB entry 3d0w coloured by chain, front view.
PDB entry 3d0w coloured by chain, side view.
PDB entry 3d0w coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Seetharaman J, Kuzin AP, Neely H, Forouhar F, Min S, Zhao L, Fang Y, Owens L, Ma L-C, Xiao R, Liu J, Baran MC, Acton TB, Rost B, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128525 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
YflH protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 104 amino acids
Theoretical weight: 12.23 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O34306 (Residues: 1-104; Coverage: 100%)
Gene names: BSU07680, yflH
Sequence domains: Protein of unknown function (DUF3243)
Structure domains: Protein of unknown function DUF3243

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A, NSLS BEAMLINE X4C
Spacegroup: P21
Unit cell:
a: 49.3Å b: 45.18Å c: 99.177Å
α: 90° β: 98.27° γ: 90°
R-values:
R R work R free
0.216 0.216 0.242
Expression system: Escherichia coli BL21

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