› 3d1h

X-ray diffraction
2.1Å resolution

Structure of the PTP-Like Phytase Expressed by Selenomonas Ruminantium at an Ionic Strength of 500 mM

Released:
DOI: 10.2210/pdb3d1h/pdb
PDB entry 3d1h coloured by chain, front view.
PDB entry 3d1h coloured by chain, side view.
PDB entry 3d1h coloured by chain, top view.
Source organism: Selenomonas ruminantium
Primary publication:
Effect of ionic strength and oxidation on the P-loop conformation of the protein tyrosine phosphatase-like phytase, PhyAsr.
Gruninger RJ, Selinger LB, Mosimann SC
FEBS J 275 3783-92 (2008)
PMID: 18573100

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182145 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine specific protein phosphatases domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 340 amino acids
Theoretical weight: 38.99 KDa
Source organism: Selenomonas ruminantium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7WUJ1 (Residues: 28-346; Coverage: 100%)
Gene name: phyA
Sequence domains: Inositol hexakisphosphate
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 45.858Å b: 137.174Å c: 79.839Å
α: 90° β: 102.97° γ: 90°
R-values:
R R work R free
0.187 0.184 0.214
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Protein tyrosine phosphatase structure-function relationships in regulation and pathogenesis.
Böhmer et al. (2013)
8 other articles

2 mentions without citation

Mechanism of the orotidine 5'-monophosphate decarboxylase-catalyzed reaction: evidence for substrate destabilization.
Chan et al. (2009)
1 more