› 3d22

X-ray diffraction
1.6Å resolution

Crystal structure of a poplar thioredoxin h mutant, PtTrxh4C61S

Released:
DOI: 10.2210/pdb3d22/pdb
PDB entry 3d22 coloured by chain, front view.
PDB entry 3d22 coloured by chain, side view.
PDB entry 3d22 coloured by chain, top view.
Source organism: Populus trichocarpa x Populus deltoides
Primary publication:
An atypical catalytic mechanism involving three cysteines of thioredoxin.
Koh CS, Navrot N, Didierjean C, Rouhier N, Hirasawa M, Knaff DB, Wingsle G, Samian R, Jacquot JP, Corbier C, Gelhaye E
J Biol Chem 283 23062-72 (2008)
PMID: 18552403

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160954 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin H-type Chain: A
Molecule details ›
Chain: A
Length: 139 amino acids
Theoretical weight: 15.68 KDa
Source organism: Populus trichocarpa x Populus deltoides
Expression system: Escherichia coli
UniProt:
  • Canonical: P85801 (Residues: 1-139; Coverage: 100%)
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P212121
Unit cell:
a: 31.776Å b: 44.103Å c: 85.678Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 0.196
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Thioredoxins and glutaredoxins: unifying elements in redox biology.
Meyer et al. (2009)
5 more

1 mention without citation

Global DNA methylation profiling reveals silencing of a secreted form of Epha7 in mouse and human germinal center B-cell lymphomas.
Dawson et al. (2007)