› 3d2s

X-ray diffraction
1.7Å resolution

Crystal structure of MBNL1 tandem zinc finger 3 and 4 domain in complex with CGCUGU RNA

Released:
DOI: 10.2210/pdb3d2s/pdb
PDB entry 3d2s coloured by chain, front view.
PDB entry 3d2s coloured by chain, side view.
PDB entry 3d2s coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into RNA recognition by the alternative-splicing regulator muscleblind-like MBNL1.
Teplova M, Patel DJ
Nat Struct Mol Biol 15 1343-51 (2008)
PMID: 19043415

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192542 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Muscleblind-like protein 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 70 amino acids
Theoretical weight: 8.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NR56 (Residues: 178-246; Coverage: 18%)
Gene names: EXP, KIAA0428, MBNL, MBNL1
Sequence domains:
Structure domains: Ribosomal Protein S8; Chain: A, domain 1
5'-R(*CP*GP*CP*UP*GP*U)-3' Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 6 nucleotides
Theoretical weight: 1.87 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 56.421Å b: 56.899Å c: 58.222Å
α: 90° β: 108.81° γ: 90°
R-values:
R R work R free
0.226 0.224 0.26
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

18 review citations

Role of RNA structure in regulating pre-mRNA splicing.
Warf et al. (2010)
17 more

17 mentions without citation

MBNL proteins and their target RNAs, interaction and splicing regulation.
Konieczny et al. (2014)
16 more