› 3d2w

X-ray diffraction
1.65Å resolution

Crystal structure of mouse TDP-43 RRM2 domain in complex with DNA

Released:
DOI: 10.2210/pdb3d2w/pdb
PDB entry 3d2w coloured by chain, front view.
PDB entry 3d2w coloured by chain, side view.
PDB entry 3d2w coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural insights into TDP-43 in nucleic-acid binding and domain interactions.
Kuo PH, Doudeva LG, Wang YT, Shen CK, Yuan HS
Nucleic Acids Res 37 1799-808 (2009)
PMID: 19174564

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-116168 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
TAR DNA-binding protein 43 Chain: A
Molecule details ›
Chain: A
Length: 89 amino acids
Theoretical weight: 10.1 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q921F2 (Residues: 192-265; Coverage: 18%)
Gene names: Tardbp, Tdp43
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
DNA (5'-D(*DGP*DTP*DTP*DGP*DAP*DGP*DCP*DGP*DTP*DT)-3') Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.09 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: F222
Unit cell:
a: 41.283Å b: 87.29Å c: 125.345Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.207 0.245
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

46 review citations

TDP-43 and FUS/TLS: emerging roles in RNA processing and neurodegeneration.
Lagier-Tourenne et al. (2010)
45 more

15 mentions without citation

Structural Insights Into TDP-43 and Effects of Post-translational Modifications.
François-Moutal et al. (2019)
14 more