› 3d45

X-ray diffraction
3Å resolution

Crystal structure of mouse PARN in complex with m7GpppG

Released:
DOI: 10.2210/pdb3d45/pdb
PDB entry 3d45 coloured by chain, front view.
PDB entry 3d45 coloured by chain, side view.
PDB entry 3d45 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis of m(7)GpppG binding to poly(A)-specific ribonuclease.
Wu M, Nilsson P, Henriksson N, Niedzwiecka A, Lim MK, Cheng Z, Kokkoris K, Virtanen A, Song H
Structure 17 276-86 (2009)
PMID: 19217398

Function and Biology Details

Reaction catalysed: 
Exonucleolytic cleavage of poly(A) to 5'-AMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186564 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(A)-specific ribonuclease PARN Chains: A, B
Molecule details ›
Chains: A, B
Length: 507 amino acids
Theoretical weight: 58.36 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8VDG3 (Residues: 1-505; Coverage: 81%)
Gene name: Parn
Sequence domains:
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

2 bound ligands:
Ligand 7MG 2 x 7MG
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 58.013Å b: 128.35Å c: 176.844Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.3 0.299 0.334
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Cap and cap-binding proteins in the control of gene expression.
Topisirovic et al. (2011)
12 more

4 mentions without citation

The diversity, plasticity, and adaptability of cap-dependent translation initiation and the associated machinery.
Borden et al. (2020)
3 more